Generation of Neural Cells by Direct Cellular Reprogramming
نویسندگان
چکیده
منابع مشابه
Generation of Neural Cells by Direct Cellular Reprogramming
Cellular reprogramming is a new technique that is currently explored for its potential to manufacture human cell types in vitro. Direct cellular reprogramming allows the direct conversion of cells into a target cell type of interest. This approach is of particular interest for cells of the central nervous system, which are notoriously difficult to obtain. Experiments in which cells were convert...
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The process of 'cell reprogramming' can be achieved by somatic cell nuclear transfer, cell fusion with embryonic stem cells, exposure to stem cell extracts, or by inducing pluripotentcy mediated by defined factors giving rise to what are termed induced pluripotent stem cells. More recently, the fate of a somatic cell can be directly induced to uptake other cell fates, termed lineage-specific re...
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Cell Culture Human newborn fibroblasts (HNF) were purchased from ATCC (CCL-117) and cultured in Dulbecco’s modified Minimal Essential Medium (DMEM, Invitrogen, Carlsbad, CA), supplemented with 2mM L-glutamine (Invitrogen, Grand island, NY). 1mM β-mercaptoethanol, 1x non-essential amino acids (NEAA; Invitrogen, Carlsbad, CA), 15% fetal bovine serum (FBS, Hyclone, Thermo Scientific, Logan UT), 10...
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Cellular reprogramming technology has created new opportunities in understanding human disease, drug discovery, and regenerative medicine. While a combinatorial code was initially found to reprogram somatic cells to pluripotency, a "second generation" of cellular reprogramming involves lineage-restricted transcription factors and microRNAs that directly reprogram one somatic cell to another. Th...
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Although it is possible to generate neural stem cells (NSC) from somatic cells by reprogramming technologies with transcription factors, clinical utilization of patient-specific NSC for the treatment of human diseases remains elusive. The risk hurdles are associated with viral transduction vectors induced mutagenesis, tumor formation from undifferentiated stem cells, and transcription factors-i...
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ژورنال
عنوان ژورنال: Journal of Transplantation & Stem Cell Biology
سال: 2014
ISSN: 2374-9326
DOI: 10.13188/2374-9326.1000001